Nitrogen generators are required for various forms of Analytical chemistry such as Liquid chromatography–mass spectrometry and Gas Chromatography where a stable and continuous supply of Nitrogen is necessary.

Although air is 78% Nitrogen, most aircraft tires are filled with nitrogen, and there are many tire and automotive shops with nitrogen generators to fill tires, the advantage being that nitrogen in a tank is dry. Often a compressed air tank will have water in it that comes from atmospheric water vapor condensing in the tank after leaving the air compressor. Also, nitrogen maintains a more stable pressure when heated and cooled and is a larger molecule (109.76 pm) so doesn't permeate the tire as easily as O2 (120.74 pm) and Argon.

In electronics, nitrogen serves as an antioxidant in the manufacture of semi-conductors and electric circuits, heat treatment of finished products, as well as in blowing and cleaning.